Histochemical and Ultrastructural Analysis of the Effect of Electromagnetic Radiation on Rat Liver and Brain

220mg of butylated hydroxytoluene/kg or l00mg of phenobarbitonelkg. Adrenalectomy diminished the initial effect of coumarin on microsomal amino acid incorporation in vitro. However, the coumarin effect on microsomal protein synthesis could not be eliminated by the removal of either the pituitary or thyroid gland. Although the doses of coumarin used in these experiments are toxic and expected to impose stress on the animals, changes in the rate of amino acid incorporation in vitro following single or repeated doses of coumarin treatment could not be entirely ascribed to stress-like phenomena. Stimulation of amino acid incorporation into protein in uitro, or in viuo, and the induced liver growth, which followed butylated hydroxytoluene and phenobarbitone treatment, were not influenced by the removal of adrenal, pituitary or thyroid glands. These results suggest that although non-toxic inducers such as butylated hydroxytoluene increase the adrenal weight, steroid or thyroid hormones do not participate in the initial triggering effect of butylated hydroxytoluene or phenobarbitone on the onset of liver enlargement. The initiation of coumarin-induced ‘toxic hepatomegaly’ however, may be mediated by some effect of the adrenal hormones.